P4HB Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55161R-100uL |
| Product Name | P4HB Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IHC
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5034 |
| Swiss Prot | P07237 |
| Subcellular location | Cytoplasm |
| Synonyms | P4HB;CLCRP1;DSI;ERBA2L;GIT;P4Hbeta;PDI;PDIA1;PHDB;PO4DB;PO4HB;PROHB |
| Background | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is P4HB Polyclonal Antibody (bs-55161R) validated for, and at what dilution?
bs-55161R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does P4HB Polyclonal Antibody (bs-55161R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55161R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise P4HB Polyclonal Antibody (bs-55161R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2).