P4HB Polyclonal Antibody

$Lane 1: THP-1 cell lysates; Lane 2: SW620 cell lysates; Lane 3: CEM cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: Raw264.7 cell lysates; Lane 6: Mouse Liver lysates; Lane 7: Mouse Spleen lysates probed with P4HB Polyclonal Antibody, Unconjugated (bs-55161) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: THP-1 cell lysates; Lane 2: SW620 cell lysates; Lane 3: CEM cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: Raw264.7 cell lysates; Lane 6: Mouse Liver lysates; Lane 7: Mouse Spleen lysates probed with P4HB Polyclonal Antibody, Unconjugated (bs-55161) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Hela cell lysates; Lane 2: P4HB knockout (KO) HeLa cell lysates probed with P4HB Polyclonal Antibody, Unconjugated (bs-55161R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: P4HB knockout (KO) HeLa cell lysates probed with P4HB Polyclonal Antibody, Unconjugated (bs-55161R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Tissue\/cell: U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P4HB) polyclonal Antibody, Unconjugated (bs-55161R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgGantibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell: U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P4HB) polyclonal Antibody, Unconjugated (bs-55161R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgGantibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P4HB) polyclonal Antibody, Unconjugated (bs-55161R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P4HB) polyclonal Antibody, Unconjugated (bs-55161R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IP
IHC
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55161R
Product Name	P4HB Polyclonal Antibody
Applications	WB IF(ICC) IP IHC IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5034
Swiss Prot	P07237
Subcellular location	Cytoplasm
Synonyms	P4HB;CLCRP1;DSI;ERBA2L;GIT;P4Hbeta;PDI;PDIA1;PHDB;PO4DB;PO4HB;PROHB
Background	This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IP	1-2ug
IHC
IF