PDCD4 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55166R |
| Product Name | PDCD4 Polyclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Specificity | KO-Validated |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2). |
| Immunogen Range | 1-260/469 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 27250 |
| Swiss Prot | Q53EL6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PDCD4;H731 |
| Background | This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:400-800 |
| IF | 1:100-500 |
| IHC-P | 1:400-800 |
Frequently asked questions
Which applications is PDCD4 Polyclonal Antibody (bs-55166R) validated for, and at what dilution?
bs-55166R is validated for WB (1:500-2000); IHC-F (1:400-800); IF (1:100-500); IHC-P (1:400-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDCD4 Polyclonal Antibody (bs-55166R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-55166R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDCD4 Polyclonal Antibody (bs-55166R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2). Immunogen range: 1-260/469.