POLE3 Polyclonal Antibody

$Lane 1: 293T cell lysates; Lane 2: POLE3 knockout (KO) 293T cell lysates probed with POLE3 Polyclonal Antibody, Unconjugated (bs-55169R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293T cell lysates; Lane 2: POLE3 knockout (KO) 293T cell lysates probed with POLE3 Polyclonal Antibody, Unconjugated (bs-55169R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: HepG2 cell lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse bone marrow lysates; Lane 4: Mouse spleen lysates probed with POLE3 Polyclonal Antibody, Unconjugated (bs-55169R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: HepG2 cell lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse bone marrow lysates; Lane 4: Mouse spleen lysates probed with POLE3 Polyclonal Antibody, Unconjugated (bs-55169R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (POLE3) polyclonal Antibody, Unconjugated (bs-55169R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.$

U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (POLE3) polyclonal Antibody, Unconjugated (bs-55169R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55169R
Product Name	POLE3 Polyclonal Antibody
Applications	WB IF(ICC) IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	54107
Swiss Prot	Q9NRF9
Subcellular location	Nucleus
Synonyms	POLE3;CHARAC17;CHRAC17;YBL1;p17
Background	OLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF