PTBP1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55181R-100uL |
| Product Name | PTBP1 Polyclonal Antibody |
| Applications |
WB
IHC
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_114368.1). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5725 |
| Swiss Prot | P26599 |
| Subcellular location | Nucleus |
| Synonyms | PTBP1;HNRNP-I;HNRNPI;HNRPI;PTB;PTB-1;PTB-T;PTB2;PTB3;PTB4;pPTB |
| Background | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is PTBP1 Polyclonal Antibody (bs-55181R) validated for, and at what dilution?
bs-55181R is validated for WB (1:300-5000); IHC (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTBP1 Polyclonal Antibody (bs-55181R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55181R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTBP1 Polyclonal Antibody (bs-55181R)?
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_114368.1).