PTPN1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55182r-bf350-100ul |
| Product Name | PTPN1 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Specificity | KO-Validated |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1). |
| Immunogen Range | 281-435/435 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5770 |
| Swiss Prot | P18031 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PTPN1;PTP1B |
| Background | The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is PTPN1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-55182R-BF350) validated for, and at what dilution?
bs-55182R-BF350 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTPN1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-55182R-BF350) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, Rabbit.
How should bs-55182R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTPN1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-55182R-BF350)?
Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1). Immunogen range: 281-435/435.
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-55182R-BF350)?
AbBy Fluor-350 on bs-55182R-BF350: excitation / emission 346nm/442nm.