RHOC Polyclonal Antibody

$Lane 1: Human U2OS cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Mouse PC-12 cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human U2OS cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Mouse PC-12 cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: 293T cell lysates; Lane 2: RHOC knockout (KO) 293T cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293T cell lysates; Lane 2: RHOC knockout (KO) 293T cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (RHOC ) polyclonal Antibody, Unconjugated (bs-55187R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (RHOC ) polyclonal Antibody, Unconjugated (bs-55187R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-55187R
Product Name	RHOC Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(ICC) IF
Specificity	KO-Validated
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
Immunogen Range	1-193/193
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	389
Swiss Prot	P08134
Subcellular location	Cell membrane
Synonyms	RHOC;ARH9;ARHC;H9;RHOH9
Background	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF