SELENBP1 Polyclonal Antibody

Lane 1: Human MCF-7 cell lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse kidney lysates probed with SELENBP1 Polyclonal Antibody, Unconjugated (bs-55195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: SELENBP1 knockout (KO) HeLa cell lysates probed with SELENBP1 Polyclonal Antibody, Unconjugated (bs-55195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SELENBP1) polyclonal Antibody, Unconjugated (bs-55195R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SELENBP1) polyclonal Antibody, Unconjugated (bs-55195R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SELENBP1 Polyclonal Antibody, Unconjugated (bs-55195R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SELENBP1 Polyclonal Antibody, Unconjugated (bs-55195R) at 1:100 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IF(ICC)
IHC
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55195R
Product Name	SELENBP1 Polyclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8991
Swiss Prot	Q13228
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	SELENBP1;HEL-S-134P;LPSB;SBP56;SP56;hSBP
Background	This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC
IF

Applications

Reactivity

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