phospho-NF1 (Ser2515) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5520R-HRP |
| Product Name | phospho-NF1 (Ser2515) Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Horse, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NF1 around the phosphorylation site of Ser2515 |
| Modification Site | Ser2515 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4763 |
| Subcellular location | Nucleus |
| Synonyms | NF1phospho S2515; p-NF1S2515; DKFZp686J1293; FLJ21220; Neurofibromatosis Noonan syndrome; Neurofibromatosis related protein NF 1; Neurofibromatosis related protein NF1; neurofibromatosis type I; Neurofibromatosis-related protein NF-1; Neurofibromin 1; Neurofibromin truncated; Neurofibromin1; NF 1; NF; NF1; NF1_HUMAN; NFNS; Type 1 Neurofibromatosis; von Recklinghausen disease neurofibromin; von Recklinghausen disease related protein VRNF; VRNF; WATS; Watson disease related protein WSS; Watson syndrome; WSS. |
| Background | Neurofibromin is a product of the tumor suppressor gene, Neurofibromatosis type I. Neurofibromin is known to have GTPase activity that modulates the ras pathway. The absence of or alteration of the neurofibromin protein may lead to Neurofibromatosis disease. This protein has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-NF1 (Ser2515) Polyclonal Antibody, HRP Conjugated (bs-5520R-HRP) validated for, and at what dilution?
bs-5520R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NF1 (Ser2515) Polyclonal Antibody, HRP Conjugated (bs-5520R-HRP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Horse, Rabbit.
How should bs-5520R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NF1 (Ser2515) Polyclonal Antibody, HRP Conjugated (bs-5520R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human NF1 around the phosphorylation site of Ser2515