UBE2O Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55219R |
| Product Name | UBE2O Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 63893 |
| Swiss Prot | Q9C0C9 |
| Subcellular location | Nucleus |
| Synonyms | UBE2O;E2-230K |
| Background | UBE20, also known as E2-230K, is a 1,292 amino acid member of the uniquitin-conjugating enzyme family that is involved in protein modification. Expressed predominately in heart and skeletal muscle, UBE2O functions to catalyze the ATP-dependent covalent attachment of ubiquitin to select proteins, thereby targeting the ubiquitinated proteins for proteasomal degradation. The gene encoding UBE2O maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IF | 1:50-200 |
Frequently asked questions
Which applications is UBE2O Polyclonal Antibody (bs-55219R) validated for, and at what dilution?
bs-55219R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBE2O Polyclonal Antibody (bs-55219R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55219R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBE2O Polyclonal Antibody (bs-55219R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).