Beta-catenin Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55228R |
| Product Name | Beta-catenin Polyclonal Antibody |
| Applications |
WB
IP
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human beta-catenin (NP_001895.1). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1499 |
| Swiss Prot | P35222 |
| Subcellular location | Nucleus, Cell membrane, Intracellular |
| Synonyms | CTNNB;MRD19;armadillo;beta Catenin;CTNNB1 |
| Background | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Beta-catenin Polyclonal Antibody (bs-55228R) validated for, and at what dilution?
bs-55228R is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Beta-catenin Polyclonal Antibody (bs-55228R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55228R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Beta-catenin Polyclonal Antibody (bs-55228R)?
A synthetic peptide corresponding to a sequence within amino acids 650-750 of human beta-catenin (NP_001895.1).