phospho-NDEL1 (Ser242) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5523R-Cy3 |
| Product Name | phospho-NDEL1 (Ser242) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NDEL1 around the phosphorylation site of Ser242 |
| Modification Site | Ser242 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81565 |
| Swiss Prot | Q9GZM8 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EOPA; NDE2; NUDEL; MITAP1; NDE1L1; Nuclear distribution protein nudE-like 1; Protein Nudel; Mitosin-associated protein 1; NDEL1 |
| Background | Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-NDEL1 (Ser242) Polyclonal Antibody, Cy3 Conjugated (bs-5523R-Cy3) validated for, and at what dilution?
bs-5523R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NDEL1 (Ser242) Polyclonal Antibody, Cy3 Conjugated (bs-5523R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5523R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NDEL1 (Ser242) Polyclonal Antibody, Cy3 Conjugated (bs-5523R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human NDEL1 around the phosphorylation site of Ser242
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5523R-Cy3)?
Cy3 on bs-5523R-Cy3: excitation / emission 512,550nm/570,615nm.