phospho-NDEL1 (Thr219) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5524R-FITC |
| Product Name | phospho-NDEL1 (Thr219) Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NDEL1 around the phosphorylation site of Thr219 |
| Modification Site | Thr219 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81565 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NDEL1phospho T219; Nudelphospho T219; A. nidulans; DKFZp451M0318; ENDOOLIGOPEPTIDASE A; EOPA; MITAP 1; MITAP1; Mitosin associated protein 1; Mitosin associated protein MITAP1; Mitosin-associated protein 1; Ndel 1; Ndel1; NDEL1_HUMAN; Nuclear distribution gene E like homolog 1; Nuclear distribution protein nudE like 1; Nuclear distribution protein nudE-like 1; NUDE like protein; NudE nuclear distribution gene E homolog like 1 A. nidulans; NudE nuclear distribution gene E homolog like 1; NUDEL; Protein Nudel. |
| Background | Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-NDEL1 (Thr219) Polyclonal Antibody, FITC Conjugated (bs-5524R-FITC) validated for, and at what dilution?
bs-5524R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NDEL1 (Thr219) Polyclonal Antibody, FITC Conjugated (bs-5524R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5524R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NDEL1 (Thr219) Polyclonal Antibody, FITC Conjugated (bs-5524R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human NDEL1 around the phosphorylation site of Thr219
What are the excitation and emission wavelengths of the FITC conjugate (bs-5524R-FITC)?
FITC on bs-5524R-FITC: excitation / emission 494nm/518nm.