phospho-NPTX1 (Tyr344) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5527R-Cy5 |
| Product Name | phospho-NPTX1 (Tyr344) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NPTX1 around the phosphorylation site of Tyr344 |
| Modification Site | Tyr344 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4884 |
| Subcellular location | Secreted |
| Synonyms | NPTX1phospho Y344; Neuronal pentraxin I; Neuronal pentraxin-1; NP 1; NP-I; NP1; Nptx1; NPTX1_HUMAN. |
| Background | NPTX1 is a member of the neuronal pentraxin gene family. Neuronal pentraxin 1 is similar to the rat NP1 gene which encodes a binding protein for the snake venom toxin taipoxin. Human NPTX1 mRNA is exclusively localized to the nervous system. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-NPTX1 (Tyr344) Polyclonal Antibody, Cy5 Conjugated (bs-5527R-Cy5) validated for, and at what dilution?
bs-5527R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NPTX1 (Tyr344) Polyclonal Antibody, Cy5 Conjugated (bs-5527R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-5527R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NPTX1 (Tyr344) Polyclonal Antibody, Cy5 Conjugated (bs-5527R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human NPTX1 around the phosphorylation site of Tyr344
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5527R-Cy5)?
Cy5 on bs-5527R-Cy5: excitation / emission 625,650nm/670nm.