Phospho-PDPK1 (Ser393) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5537R-Cy3 |
| Product Name | Phospho-PDPK1 (Ser393) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser393 |
| Modification Site | Ser393 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5170 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PDPK1phospho S393; 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN. |
| Background | PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PDPK1 (Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-5537R-Cy3) validated for, and at what dilution?
bs-5537R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PDPK1 (Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-5537R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-5537R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PDPK1 (Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-5537R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser393
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5537R-Cy3)?
Cy3 on bs-5537R-Cy3: excitation / emission 512,550nm/570,615nm.