phospho-PAK1 (Thr212) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5542R-Cy5-100uL |
| Product Name | phospho-PAK1 (Thr212) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr212 |
| Modification Site | Thr212 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5058 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PAK1Thr212; PAK1phospho T212; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein Cdc42/Rac activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 yeast Ste20 related; p21/Cdc42/Rac1 activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 STE20 homolog, yeast; p65 PAK; p65-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast; STE20 homolog yeast. |
| Background | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PAK1 (Thr212) Polyclonal Antibody, Cy5 Conjugated (bs-5542R-Cy5) validated for, and at what dilution?
bs-5542R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PAK1 (Thr212) Polyclonal Antibody, Cy5 Conjugated (bs-5542R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Rabbit.
How should bs-5542R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PAK1 (Thr212) Polyclonal Antibody, Cy5 Conjugated (bs-5542R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr212
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5542R-Cy5)?
Cy5 on bs-5542R-Cy5: excitation / emission 625,650nm/670nm.