phospho-PRKD3 (Ser41) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5564R-Cy3 |
| Product Name | phospho-PRKD3 (Ser41) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PRKD3 around the phosphorylation site of Ser41 [RL(p-S)NG] |
| Modification Site | Ser41 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PRKD3phospho S41; PKD/PKC_Phospho-Ser41; EPK 2; EPK2; EPK-2; nPKC nu; nPKCnu; nu; PKCnu; PKD 3; PKD3; PRK D3; PRKCN; PRKD 3; PRKD3; Protein kinase C; Protein kinase C nu; Protein kinase C nu type; Protein kinase D3; Protein kinase EPK 2; Protein kinase EPK2; Serine threonine protein kinase; Serine/threonine protein kinase D3. |
| Background | Protein kinase C (PKC) is a family of serine and threonine specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PRKD3 (Ser41) Polyclonal Antibody, Cy3 Conjugated (bs-5564R-Cy3) validated for, and at what dilution?
bs-5564R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PRKD3 (Ser41) Polyclonal Antibody, Cy3 Conjugated (bs-5564R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Rabbit.
How should bs-5564R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PRKD3 (Ser41) Polyclonal Antibody, Cy3 Conjugated (bs-5564R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PRKD3 around the phosphorylation site of Ser41 [RL(p-S)NG]
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5564R-Cy3)?
Cy3 on bs-5564R-Cy3: excitation / emission 512,550nm/570,615nm.