phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5570R-Cy3 |
| Product Name | phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317 |
| Modification Site | Tyr317 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5106 |
| Swiss Prot | Q16822 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PEPCK; PEPCK2; PEPCK-M; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PCK2 |
| Background | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated (bs-5570R-Cy3) validated for, and at what dilution?
bs-5570R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated (bs-5570R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-5570R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PI3KCA (Tyr317) Polyclonal Antibody, Cy3 Conjugated (bs-5570R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5570R-Cy3)?
Cy3 on bs-5570R-Cy3: excitation / emission 512,550nm/570,615nm.