PTPN6(Tyr536) Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with PTPN6(Tyr536) Polyclonal Antibody (bs-5577R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with PTPN6(Tyr536) Polyclonal Antibody (bs-5577R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Dog
Horse

Overview
Catalog #	bs-5577R
Product Name	PTPN6(Tyr536) Polyclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Dog, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 [SE(p-Y)GN]
Modification Site	Tyr536
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5777
Swiss Prot	P29350
Subcellular location	Cytoplasm, Nucleus
Synonyms	SHP-1Tyr536; PTPN6phospho Y536; SHP1phospho Y536; 70 kda SHP1L protein; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; PTP1C; PTPN6; SH PTP 1; SHP1; SHP1L; PTN6_HUMAN.
Background	SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products