phospho-PRKCQ (Ser676) Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5583R-Cy3 |
| Product Name | phospho-PRKCQ (Ser676) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PRKCQ around the phosphorylation site of Ser676 |
| Modification Site | Ser676 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5588 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PRKCQphospho S676; PKCSer676; PKC thetaphospho S676; PKC 0; PRKCQ; PKC0; Prkcq; PRKCT; Protein kinase C theta; Protein kinase C theta type; Protein Kinase Ctheta; KPCT_HUMAN; nPKC theta; nPKC-theta; nPKCtheta. |
| Background | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PRKCQ (Ser676) Polyclonal Antibody, Cy3 Conjugated (bs-5583R-Cy3) validated for, and at what dilution?
bs-5583R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5583R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PRKCQ (Ser676) Polyclonal Antibody, Cy3 Conjugated (bs-5583R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PRKCQ around the phosphorylation site of Ser676
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5583R-Cy3)?
Cy3 on bs-5583R-Cy3: excitation / emission 512,550nm/570,615nm.