phospho-14-3-3 epsilon (Thr232) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5590R-Cy7 |
| Product Name | phospho-14-3-3 epsilon (Thr232) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human 14-3-3 around the phosphorylation site of Thr232 |
| Modification Site | Thr232 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7531 |
| Subcellular location | Nucleus |
| Synonyms | 14-3-3, mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, tyrosine 3/tryptophan 5 -monooxygenase activation protein. |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-14-3-3 epsilon (Thr232) Polyclonal Antibody, Cy7 Conjugated (bs-5590R-Cy7) validated for, and at what dilution?
bs-5590R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-14-3-3 epsilon (Thr232) Polyclonal Antibody, Cy7 Conjugated (bs-5590R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep.
How should bs-5590R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-14-3-3 epsilon (Thr232) Polyclonal Antibody, Cy7 Conjugated (bs-5590R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human 14-3-3 around the phosphorylation site of Thr232
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5590R-Cy7)?
Cy7 on bs-5590R-Cy7: excitation / emission 743nm/767nm.