phospho-YES1 (Tyr537) Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5592R-Cy7 |
| Product Name | phospho-YES1 (Tyr537) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human YES1 around the phosphorylation site of Tyr537:PQ(p-Y)QP? |
| Modification Site | Tyr537 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10413 |
| Swiss Prot | P07947 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | YES1phospho Y537; c Yes; c-Yes; Cellular Yes 1 protein; Cellular Yes1 protein; HsT441; P61 YES; p61-Yes; Proto oncogene tyrosine protein kinase YES; Proto-oncogene c-Yes; Proto-oncogene tyrosine-protein kinase Yes; v yes 1 Yamaguchi sarcoma viral oncogene homolog 1; Viral oncogene yes 1 homolog 1; Viral oncogene yes1 homolog 1; Yamaguchi sarcoma oncogene; Yes 1; Yes; YES_HUMAN; YES1. |
| Background | This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-YES1 (Tyr537) Polyclonal Antibody, Cy7 Conjugated (bs-5592R-Cy7) validated for, and at what dilution?
bs-5592R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5592R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-YES1 (Tyr537) Polyclonal Antibody, Cy7 Conjugated (bs-5592R-Cy7)?
KLH conjugated Synthesised phosphopeptide derived from human YES1 around the phosphorylation site of Tyr537:PQ(p-Y)QP?
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5592R-Cy7)?
Cy7 on bs-5592R-Cy7: excitation / emission 743nm/767nm.