phospho-PTK9 (Tyr321) Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5613R-Biotin |
| Product Name | phospho-PTK9 (Tyr321) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321 |
| Modification Site | Tyr321 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 5756 |
| Subcellular location | Cytoplasm |
| Synonyms | PTK9 Tyr321; PTK9 Y321; p-PTK9 Tyr321; p-TWF1Y321; A6; A6 protein tyrosine kinase; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; TWF1; Twinfilin 1; Twinfilin actin binding protein, homolog 1; Twinfilin; Twinfilin, actin-binding protein, homolog 1 Drosophila; Twinfilin, Drosophila, homolog of, 1; TWF1_HUMAN; Twinfilin-1. |
| Background | PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-PTK9 (Tyr321) Polyclonal Antibody, Biotin Conjugated (bs-5613R-Biotin) validated for, and at what dilution?
bs-5613R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5613R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PTK9 (Tyr321) Polyclonal Antibody, Biotin Conjugated (bs-5613R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321