phospho-PTK9 (Tyr321) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5613R-Cy5 |
| Product Name | phospho-PTK9 (Tyr321) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321 |
| Modification Site | Tyr321 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5756 |
| Subcellular location | Cytoplasm |
| Synonyms | PTK9 Tyr321; PTK9 Y321; p-PTK9 Tyr321; p-TWF1Y321; A6; A6 protein tyrosine kinase; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; TWF1; Twinfilin 1; Twinfilin actin binding protein, homolog 1; Twinfilin; Twinfilin, actin-binding protein, homolog 1 Drosophila; Twinfilin, Drosophila, homolog of, 1; TWF1_HUMAN; Twinfilin-1. |
| Background | PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PTK9 (Tyr321) Polyclonal Antibody, Cy5 Conjugated (bs-5613R-Cy5) validated for, and at what dilution?
bs-5613R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5613R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PTK9 (Tyr321) Polyclonal Antibody, Cy5 Conjugated (bs-5613R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5613R-Cy5)?
Cy5 on bs-5613R-Cy5: excitation / emission 625,650nm/670nm.