phospho-TRIM25 (The91) Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5614R-Cy7 |
| Product Name | phospho-TRIM25 (The91) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91 |
| Modification Site | Thr91 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7706 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | TRIM25phospho T91_EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite mot containing 25; Tripartite mot containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 estrogen responsive finger protein; ZNF 147; ZNF147. |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-TRIM25 (The91) Polyclonal Antibody, Cy7 Conjugated (bs-5614R-Cy7) validated for, and at what dilution?
bs-5614R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5614R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TRIM25 (The91) Polyclonal Antibody, Cy7 Conjugated (bs-5614R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5614R-Cy7)?
Cy7 on bs-5614R-Cy7: excitation / emission 743nm/767nm.