phospho-TRIM25 (Ser100) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5615R-Biotin |
| Product Name | phospho-TRIM25 (Ser100) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100 |
| Modification Site | Ser100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 7706 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | TRIM25phospho S100_EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite mot containing 25; Tripartite mot containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 estrogen responsive finger protein; ZNF 147; ZNF147. |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-TRIM25 (Ser100) Polyclonal Antibody, Biotin Conjugated (bs-5615R-Biotin) validated for, and at what dilution?
bs-5615R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-TRIM25 (Ser100) Polyclonal Antibody, Biotin Conjugated (bs-5615R-Biotin) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5615R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TRIM25 (Ser100) Polyclonal Antibody, Biotin Conjugated (bs-5615R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100