phospho-STAT5a (Ser780) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5620R-Cy3 |
| Product Name | phospho-STAT5a (Ser780) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Ser780 |
| Modification Site | Ser780 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6776 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | STAT5ASer780; STAT5Aphospho S780; Mammary gland factor; Mammary gland factor; MGF; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5A; STA5A_HUMAN; STAT 5; STAT 5A; STAT5; STAT5A. |
| Background | Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-STAT5a (Ser780) Polyclonal Antibody, Cy3 Conjugated (bs-5620R-Cy3) validated for, and at what dilution?
bs-5620R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-STAT5a (Ser780) Polyclonal Antibody, Cy3 Conjugated (bs-5620R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse.
How should bs-5620R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-STAT5a (Ser780) Polyclonal Antibody, Cy3 Conjugated (bs-5620R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Ser780
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5620R-Cy3)?
Cy3 on bs-5620R-Cy3: excitation / emission 512,550nm/570,615nm.