Phospho-STMN1 (Ser25) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5623R-Cy7 |
| Product Name | Phospho-STMN1 (Ser25) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Stathmin around the phosphorylation site of Ser25 |
| Modification Site | Ser25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3925 |
| Subcellular location | Cytoplasm, Extracellular matrix |
| Synonyms | Stathminphospho S25; Lag ; LAP 18 ; LAP18 ; Leukemia associated phosphoprotein p18 ; Metablastin ; Oncoprotein 18 ; OP 18 ; OP18 ; p18 ; p19 ; Phosphoprotein 19 ; Phosphoprotein p19 ; PP17 ; PP19 ; PR22 ; Pr22 protein ; Prosolin ; Protein Pr22 ; SMN ; Stathmin ; Stathmin1 ; STMN 1 ; STMN1. |
| Background | This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-STMN1 (Ser25) Polyclonal Antibody, Cy7 Conjugated (bs-5623R-Cy7) validated for, and at what dilution?
bs-5623R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-STMN1 (Ser25) Polyclonal Antibody, Cy7 Conjugated (bs-5623R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-5623R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-STMN1 (Ser25) Polyclonal Antibody, Cy7 Conjugated (bs-5623R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human Stathmin around the phosphorylation site of Ser25
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5623R-Cy7)?
Cy7 on bs-5623R-Cy7: excitation / emission 743nm/767nm.