phospho-Alpha synuclein (Ser129) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5628R-PerCP-Cy5.5-100uL |
| Product Name | phospho-Alpha synuclein (Ser129) Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SNCA around the phosphorylation site of Ser129 [MP(p-S)EE] |
| Modification Site | Ser129 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6622 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | SNCAphospho S129; alpha Synuclein phospho S129 ;alpha-Synuclein Ser129; Synuclein alphaSer129; Alpha synuclein; Alpha-Synuclein; Alpha-synuclein, isoform NACP140; alpha SYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid , precursor of; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease autosomal dominant, Lewy body 4; Parkinson disease familial 1; PD 1; PD1; Syn; SNCA; Snca synuclein, alpha non A4 component of amyloid precursor; SYUA_HUMAN; Synuclein alpha; Synuclein, alpha non A4 component of amyloid precursor; |
| Background | The synucleins, including Alpha-synuclein (also designated NACP for nonamyloid component precursor),Beta-synuclein (also designated PNP 14 for neuroprotein 14)and Gamma-synuclein (also designated persyn or BCSG1 for breast cancer-specific gene 1)are presynaptic protein abundant in neurons. Alpha-synuclein, a component of Alzheimer?s disease amyloid plaques, is localized to neuronal cell bodies and synapses. Coordinate expression of Alpha-synucleinand Beta-synuclein may be important during hematopoetic cell differentiation. In patients with Parkinson?s disease, a mutant form of Alpha-synuclein has been found and Gamma-synuclein is associated with axonal pathology. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Alpha synuclein (Ser129) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-5628R-PERCP-CY5-5) validated for, and at what dilution?
BS-5628R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Alpha synuclein (Ser129) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-5628R-PERCP-CY5-5) react with?
Tested reactivity: Human.
How should BS-5628R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Alpha synuclein (Ser129) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-5628R-PERCP-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human SNCA around the phosphorylation site of Ser129 [MP(p-S)EE]
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-5628R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-5628R-PERCP-CY5-5: excitation / emission 482nm/694nm.