phospho-RAD9(Ser336) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5655R-BF350 |
| Product Name | phospho-RAD9(Ser336) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RAD9 around the phosphorylation site of Ser336 [PH(p-S)EE] |
| Modification Site | Ser336 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5883 |
| Subcellular location | Nucleus |
| Synonyms | RAD9Aphospho S336; Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 S pombe homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN. |
| Background | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RAD9(Ser336) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5655R-BF350) validated for, and at what dilution?
bs-5655R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-RAD9(Ser336) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5655R-BF350) react with?
Tested reactivity: Human.
How should bs-5655R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RAD9(Ser336) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5655R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human RAD9 around the phosphorylation site of Ser336 [PH(p-S)EE]
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-5655R-BF350)?
AbBy Fluor-350 on bs-5655R-BF350: excitation / emission 346nm/442nm.