phospho-RAD9 (Tyr28) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5659R-Cy5.5-100uL |
| Product Name | phospho-RAD9 (Tyr28) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28 [EL(p-Y)LE] |
| Modification Site | Tyr28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5883 |
| Subcellular location | Nucleus |
| Synonyms | RAD9Aphospho Y28; Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 S pombe homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN. |
| Background | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RAD9 (Tyr28) Polyclonal Antibody, Cy5.5 Conjugated (BS-5659R-CY5-5) validated for, and at what dilution?
BS-5659R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-RAD9 (Tyr28) Polyclonal Antibody, Cy5.5 Conjugated (BS-5659R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Horse, Rabbit.
How should BS-5659R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RAD9 (Tyr28) Polyclonal Antibody, Cy5.5 Conjugated (BS-5659R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28 [EL(p-Y)LE]
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5659R-CY5-5)?
Cy5.5 on BS-5659R-CY5-5: excitation / emission 675nm/694nm.