phospho-NFKB p65 (Thr505) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5663R-FITC-100uL |
| Product Name | phospho-NFKB p65 (Thr505) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser301 |
| Modification Site | Thr505 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5970 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NF B-p65 Thr505; NF B-p65 Thr505; RELAphospho T505; NFKBp65; NF-Bp65; p65 NF kappaB; p65 NFkB; NFKBp65; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B cells 3 p65; V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A avian; v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; Avian reticuloendotheliosis viral v rel oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells. |
| Background | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-NFKB p65 (Thr505) Polyclonal Antibody, FITC Conjugated (bs-5663R-FITC) validated for, and at what dilution?
bs-5663R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NFKB p65 (Thr505) Polyclonal Antibody, FITC Conjugated (bs-5663R-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig.
How should bs-5663R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NFKB p65 (Thr505) Polyclonal Antibody, FITC Conjugated (bs-5663R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser301
What are the excitation and emission wavelengths of the FITC conjugate (bs-5663R-FITC)?
FITC on bs-5663R-FITC: excitation / emission 494nm/518nm.