phospho-RPS6KB1 (Thr412) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5669R-BF405-100uL |
| Product Name | phospho-RPS6KB1 (Thr412) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Thr412 |
| Modification Site | Thr 412 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6198 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | RPS6KB1 phospho S412; RPS6KB1 phospho Ser412; p-RPS6KB1 Ser412; RPS6KB1; PS6K; S6K; S6K1; STK14A; p70S6K-alpha; p70-S6K; p70-alpha; ribosomal protein S6 kinase; 70kDa; p70 S6 Kinase; P70 Beta1; KS6B1_HUMAN; Ribosomal protein S6 kinase beta-1; S6K-beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA. |
| Background | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RPS6KB1 (Thr412) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5669R-BF405) validated for, and at what dilution?
bs-5669R-BF405 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-RPS6KB1 (Thr412) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5669R-BF405) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken.
How should bs-5669R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RPS6KB1 (Thr412) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5669R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Thr412
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5669R-BF405)?
AbBy Fluor-405 on bs-5669R-BF405: excitation / emission 401nm/421nm.