phospho-ROCK2 (Thr249) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5675R-APC-Cy7-100uL |
| Product Name | phospho-ROCK2 (Thr249) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249 |
| Modification Site | Thr249 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9475 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ROCK2phospho T249; p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN. |
| Background | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ROCK2 (Thr249) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5675R-APC-Cy7) validated for, and at what dilution?
bs-5675R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ROCK2 (Thr249) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5675R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.
How should bs-5675R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ROCK2 (Thr249) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5675R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-5675R-APC-Cy7)?
APC-Cy7 on bs-5675R-APC-Cy7: excitation / emission 650nm/780nm.