Phospho-RASGRF1 (Ser929) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5683R-APC-100uL |
| Product Name | Phospho-RASGRF1 (Ser929) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser929 |
| Modification Site | Ser929 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5923 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | RASGRF1phospho S929; RASGRF1phospho Ser929; p-RASGRF1S929; p-RASGRF1Ser929; CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; HGRF55; PP13187; Ras protein specic guanine nucleotide releasing factor 1; Ras specic guanine nucleotide releasing factor; Ras specic guanine nucleotide releasing factor CDC25 homolog; Ras specic nucleotide exchange factor CDC25. |
| Background | The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-RASGRF1 (Ser929) Polyclonal Antibody, APC Conjugated (bs-5683R-APC) validated for, and at what dilution?
bs-5683R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-RASGRF1 (Ser929) Polyclonal Antibody, APC Conjugated (bs-5683R-APC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Chicken.
How should bs-5683R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-RASGRF1 (Ser929) Polyclonal Antibody, APC Conjugated (bs-5683R-APC)?
KLH conjugated synthetic phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser929
What are the excitation and emission wavelengths of the APC conjugate (bs-5683R-APC)?
APC on bs-5683R-APC: excitation / emission 650nm/660nm.