phospho-RGS19 (Ser24) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5687R-Cy5-100uL |
| Product Name | phospho-RGS19 (Ser24) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RGS19 around the phosphorylation site of Ser24 [M(p-S)SH] |
| Modification Site | Ser24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10287 |
| Subcellular location | Cell membrane |
| Synonyms | RGS19phospho S24; RGS19phospho Ser24; G alpha interacting protein; GAIP; GNAI3IP; Guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; Regulator of G protein signalling 19; RGSGAIP; RGS19_HUMAN; Regulator of G-protein signaling 19; RGS19; G-alpha-interacting protein. |
| Background | G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase activating protein that functions to down regulate Galpha i/Galpha q linked signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RGS19 (Ser24) Polyclonal Antibody, Cy5 Conjugated (bs-5687R-Cy5) validated for, and at what dilution?
bs-5687R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5687R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RGS19 (Ser24) Polyclonal Antibody, Cy5 Conjugated (bs-5687R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human RGS19 around the phosphorylation site of Ser24 [M(p-S)SH]
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5687R-Cy5)?
Cy5 on bs-5687R-Cy5: excitation / emission 625,650nm/670nm.