phospho-RPH3AL (Ser232) Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5695R-Cy7 |
| Product Name | phospho-RPH3AL (Ser232) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human rab effector Noc2 isoform 2 around the phosphorylation site of Ser232 [HL(p-S)GC] |
| Modification Site | Ser232 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9501 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Rph3aphospho S232; Noc2; Rab effector Noc2; Rabphilin 3A-like without C2 domains; Rabphilin 3A-like; Rabphilin-3A-like protein; RPH3AL; RPH3L_HUMAN; No C2 domains protein; Gm1753. |
| Background | Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells (By similarity). Acts as a potential RAB3B effector protein in epithelial cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RPH3AL (Ser232) Polyclonal Antibody, Cy7 Conjugated (bs-5695R-Cy7) validated for, and at what dilution?
bs-5695R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5695R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RPH3AL (Ser232) Polyclonal Antibody, Cy7 Conjugated (bs-5695R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human rab effector Noc2 isoform 2 around the phosphorylation site of Ser232 [HL(p-S)GC]
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5695R-Cy7)?
Cy7 on bs-5695R-Cy7: excitation / emission 743nm/767nm.