phospho-SCNN1B (Ser633) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5702R-HRP |
| Product Name | phospho-SCNN1B (Ser633) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-F
|
| Reactivity | Human, Mouse, GuineaPig |
| Predicted Reactivity | Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human SCNN1B around the phosphorylation site of Ser633 |
| Modification Site | Ser633 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6338 |
| Swiss Prot | P51168 |
| Subcellular location | Cell membrane |
| Synonyms | BESC1; ENaCb; SCNEB; ENaCbeta; Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na(+) channel subunit beta; Beta-ENaC; Nonvoltage-gated sodium channel 1 subunit beta; SCNN1B |
| Background | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-SCNN1B (Ser633) Polyclonal Antibody, HRP Conjugated (bs-5702R-HRP) validated for, and at what dilution?
bs-5702R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-SCNN1B (Ser633) Polyclonal Antibody, HRP Conjugated (bs-5702R-HRP) react with?
Tested reactivity: Human, Mouse, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig.
How should bs-5702R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-SCNN1B (Ser633) Polyclonal Antibody, HRP Conjugated (bs-5702R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human SCNN1B around the phosphorylation site of Ser633