phospho-STAT5b (Ser731) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5703R-BF594 |
| Product Name | phospho-STAT5b (Ser731) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human STAT5b around the phosphorylation site of Ser731 |
| Modification Site | Ser731 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6777 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | STAT5bphospho S731; Transcription factor STAT5B; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5; Stat5b. |
| Background | Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-STAT5b (Ser731) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-5703R-BF594) validated for, and at what dilution?
bs-5703R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-STAT5b (Ser731) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-5703R-BF594) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-5703R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-STAT5b (Ser731) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-5703R-BF594)?
KLH conjugated synthetic phosphopeptide derived from human STAT5b around the phosphorylation site of Ser731
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-5703R-BF594)?
AbBy Fluor-594 on bs-5703R-BF594: excitation / emission 590nm/617nm.