BubR1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5726R-Cy5.5-100uL |
| Product Name | BubR1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BubR1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 701 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Beta homolg of S. cerevisiae BUB 1; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles; BUB 1B; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; Bub1A; BUB1B; BUB1B_HUMAN; BUB1beta; BUBR1; Budding Uninhibited by Benzimidazoles 1 beta; hBUBR1; MAD3/BUB1 related protein kinase; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint gene BUB1B; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Protein SSK1; SSK 1; SSK1. |
| Background | BubR1 |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BubR1 Polyclonal Antibody, Cy5.5 Conjugated (BS-5726R-CY5-5) validated for, and at what dilution?
BS-5726R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BubR1 Polyclonal Antibody, Cy5.5 Conjugated (BS-5726R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Rabbit.
How should BS-5726R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BubR1 Polyclonal Antibody, Cy5.5 Conjugated (BS-5726R-CY5-5)?
KLH conjugated synthetic peptide derived from human BubR1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5726R-CY5-5)?
Cy5.5 on BS-5726R-CY5-5: excitation / emission 675nm/694nm.