HURP Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5728R-Cy7 |
| Product Name | HURP Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HURP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1982 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DAP-5; DAP5; Discs large homolog 7; Disks large associated protein 5; Disks large associated protein DLG7; Disks large-associated protein 5; Disks large-associated protein DLG7; DLG 1; DLG 7; DLG1; DLG7; DLGAP5; DLGP5_HUMAN; Drosophila discs large 1 tumor suppressor like; Hepatoma up regulated protein; Hepatoma up-regulated protein; Hepatoma upregulated protein; HURP. |
| Background | Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HURP Polyclonal Antibody, Cy7 Conjugated (bs-5728R-Cy7) validated for, and at what dilution?
bs-5728R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HURP Polyclonal Antibody, Cy7 Conjugated (bs-5728R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit.
How should bs-5728R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HURP Polyclonal Antibody, Cy7 Conjugated (bs-5728R-Cy7)?
KLH conjugated synthetic peptide derived from human HURP
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5728R-Cy7)?
Cy7 on bs-5728R-Cy7: excitation / emission 743nm/767nm.