Mad2L1 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5731R-Cy3-100uL |
| Product Name | Mad2L1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Mad2L1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4085 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HsMAD 2; MAD 2; MAD 2A; MAD2 like 1; MAD2 like protein 1; MAD2 mitotic arrest deficient like 1; Mitotic arrest deficient yeast homolog like 1; MD2L1_HUMAN. |
| Background | Mad2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Mad2L1 Polyclonal Antibody, Cy3 Conjugated (bs-5731R-Cy3) validated for, and at what dilution?
bs-5731R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5731R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mad2L1 Polyclonal Antibody, Cy3 Conjugated (bs-5731R-Cy3)?
KLH conjugated synthetic peptide derived from human Mad2L1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5731R-Cy3)?
Cy3 on bs-5731R-Cy3: excitation / emission 512,550nm/570,615nm.