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Cyclin A1 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5739R-RBITC
Product Name Cyclin A1 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cyclin A1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8900
Swiss Prot P78396
Subcellular location Nucleus
Synonyms CT146; Cyclin-A1; CCNA1
Background May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cyclin A1 Polyclonal Antibody, RBITC Conjugated (bs-5739R-RBITC) validated for, and at what dilution?

bs-5739R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cyclin A1 Polyclonal Antibody, RBITC Conjugated (bs-5739R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Rabbit.

How should bs-5739R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cyclin A1 Polyclonal Antibody, RBITC Conjugated (bs-5739R-RBITC)?

KLH conjugated synthetic peptide derived from human Cyclin A1

What are the excitation and emission wavelengths of the RBITC conjugate (bs-5739R-RBITC)?

RBITC on bs-5739R-RBITC: excitation / emission 540nm/625nm.