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phospho-MARK2 (Ser595) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tMARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tMARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (bs-0295G-Cy3) at 1:500 for 90 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (bs-0295G-Cy3) at 1:500 for 90 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MARK2(Ser595) Polyclonal Antibody, Unconjugated (bs-5742R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-5742r
Product Name phospho-MARK2 (Ser595) Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human MARK2 around the phosphorylation site of Ser595
Immunogen Range 570-610/788
Modification Site Ser595
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 2011
Swiss Prot Q7KZI7
Synonyms ELKL mot kinase 1; MARK2phospho S595; ELKL mot kinase; EMK-1; EMK1; MAP/microtubule affinity regulating kinase 2; MAP/microtubule affinity-regulating kinase 2; Mark2; MARK2_HUMAN; PAR1 homolog; Serine/threonine protein kinase MARK2; Serine/threonine-protein kinase MARK2.
Background MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule.stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
Application Dilution
WB
IHC-P
IHC-F
IF

Frequently asked questions

Which applications is phospho-MARK2 (Ser595) Polyclonal Antibody (bs-5742R) validated for, and at what dilution?

bs-5742R is validated for WB, IHC-P, IHC-F, IF.

Which species does phospho-MARK2 (Ser595) Polyclonal Antibody (bs-5742R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-5742R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-MARK2 (Ser595) Polyclonal Antibody (bs-5742R)?

KLH conjugated synthetic phosphopeptide derived from human MARK2 around the phosphorylation site of Ser595 Immunogen range: 570-610/788.

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