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CKS1 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-5745R-BF555-100uL
Product Name CKS1 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CKS1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1163
Subcellular location Nucleus
Synonyms CDC2 associated protein CKS1; CDC28 protein kinase 1; CDC28 protein kinase 1B; CDC28 protein kinase regulatory subunit 1B; Cell division control protein CKS1; CKS 1; CKS1B; Ckshs1; Cyclin dependent kinases regulatory subunit 1; NB4 apoptosis/dferentiation related protein; PNAS 143; PNAS 16; PNAS 18.
Background CKS1 protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CKS1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-5745R-BF555) validated for, and at what dilution?

bs-5745R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-5745R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CKS1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-5745R-BF555)?

KLH conjugated synthetic peptide derived from human CKS1

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-5745R-BF555)?

AbBy Fluor-555 on bs-5745R-BF555: excitation / emission 553nm/568nm.

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