Epigen Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5767R-PerCP-Cy5.5-100uL |
| Product Name | Epigen Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Epigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 255324 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | EPG; Epigen precursor; Epithelial mitogen; EPGN_HUMAN. |
| Background | Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Epigen Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-5767R-PERCP-CY5-5) validated for, and at what dilution?
BS-5767R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-5767R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Epigen Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-5767R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human Epigen
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-5767R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-5767R-PERCP-CY5-5: excitation / emission 482nm/694nm.