KLST/Kallistatin Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5786R-BF750-100uL |
| Product Name | KLST/Kallistatin Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLST/Kallistatin (161-220aa) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5267 |
| Subcellular location | Secreted |
| Synonyms | Serpin peptidase inhibitor clade A member 4; Kallikrein binding protein; Kallikrein inhibitor; Protease inhibitor 4; Serpin A4; KAL; Kallikrein binding protein; Kallikrein inhibitor; KBP; KLST; KST; MGC108582; PI4; Protease inhibitor 4; Serpin A4; Serpin peptidase inhibitor clade A member 4; SERPINA4; KAIN_HUMAN. |
| Background | Kallistatin is a serine proteinase inhibitor which binds to tissue kallikrein and inhibits its amidolytic and kininogenase activity. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KLST/Kallistatin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5786R-BF750) validated for, and at what dilution?
bs-5786R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KLST/Kallistatin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5786R-BF750) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-5786R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KLST/Kallistatin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5786R-BF750)?
KLH conjugated synthetic peptide derived from human KLST/Kallistatin (161-220aa)
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-5786R-BF750)?
AbBy Fluor-750 on bs-5786R-BF750: excitation / emission 749nm/775nm.