ELMO1 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5807R-APC-Cy5.5-100uL |
| Product Name | ELMO1 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ELMO. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9844 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CED 12; Ced 12 homolog 1; Ced 12 homolog; CED12; Ced12 homolog 1; Ced12 homolog; ELMO 1; Engulfment and cell motility 1; Engulfment and cell motility protein 1; KIAA0281; ELMO1_HUMAN. |
| Background | ELMO1 is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in association with DOCK1 and CRK and was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. It is belived to enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ELMO1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5807R-APC-CY5-5) validated for, and at what dilution?
BS-5807R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-5807R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ELMO1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5807R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human ELMO.
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-5807R-APC-CY5-5)?
APC-Cy5.5 on BS-5807R-APC-CY5-5: excitation / emission 650nm/694nm.