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PRODH Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Horse
Overview
Catalog # bs-5813R-APC-100uL
Product Name PRODH Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Horse
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PRODH
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5625
Swiss Prot O43272
Subcellular location Cytoplasm
Synonyms POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6; Proline dehydrogenase 1, mitochondrial; Proline oxidase; Proline oxidase 2; p53-induced gene 6 protein; PRODH; POX2
Background Proline oxidase catalyzes the conversion of proline to pyrroline-5-carboxylate, or P5C during the degradation of the amino acid Proline. Defects in PRODH are the cause of hyperprolinemia type 1, a disorder characterized by elevated serum proline levels. Defective PRODH may be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome and may be associated with susceptibility to schizophrenia 4 (SCZD4).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PRODH Polyclonal Antibody, APC Conjugated (bs-5813R-APC) validated for, and at what dilution?

bs-5813R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PRODH Polyclonal Antibody, APC Conjugated (bs-5813R-APC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Horse.

How should bs-5813R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PRODH Polyclonal Antibody, APC Conjugated (bs-5813R-APC)?

KLH conjugated synthetic peptide derived from human PRODH

What are the excitation and emission wavelengths of the APC conjugate (bs-5813R-APC)?

APC on bs-5813R-APC: excitation / emission 650nm/660nm.