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RAB21 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-5830R-Cy7
Product Name RAB21 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RAB21.
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23011
Swiss Prot Q9UL25
Subcellular location Cytoplasm
Synonyms Ras-related protein Rab-21; RAB21; KIAA0118
Background Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Involved in neurite growth (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RAB21 Polyclonal Antibody, Cy7 Conjugated (bs-5830R-Cy7) validated for, and at what dilution?

bs-5830R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RAB21 Polyclonal Antibody, Cy7 Conjugated (bs-5830R-Cy7) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Horse, Chicken, Rabbit.

How should bs-5830R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RAB21 Polyclonal Antibody, Cy7 Conjugated (bs-5830R-Cy7)?

KLH conjugated synthetic peptide derived from human RAB21.

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5830R-Cy7)?

Cy7 on bs-5830R-Cy7: excitation / emission 743nm/767nm.