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ASAP3 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-5832R-HRP
Product Name ASAP3 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ASAP3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 1; ACAP4; Arf GAP with SH3 domain; Arf GAP with SH3 domain, ANK repeat and PH domain containing protein 3; ARF6 GTPase activating protein; ArfGAP with SH3 domain, ankyrin repeat and PH domain; Centaurin, beta 6; CENTB6; DDEFL1; Development and dferentiation enhancing factor like 1; Up regulated in liver cancer 1 UPLC1; UPLC1.
Background ASAP3 is a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is ASAP3 Polyclonal Antibody, HRP Conjugated (bs-5832R-HRP) validated for, and at what dilution?

bs-5832R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ASAP3 Polyclonal Antibody, HRP Conjugated (bs-5832R-HRP) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.

How should bs-5832R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ASAP3 Polyclonal Antibody, HRP Conjugated (bs-5832R-HRP)?

KLH conjugated synthetic peptide derived from human ASAP3